Transcript analysis in drug-free conditions.

<p>(A) Relative quantitative expression of the <i>rna</i>III and <i>wal</i>K regulator genes and (B) of some autolytic (<i>atl</i>, <i>lyt</i>M), cell-wall turnover (<i>sce</i>D) and cell envelope charge genes (<i>mpr</i>F, <i>dlt</i>A) in hVISA and VISA. Statistically significant difference between sample <i>vs.</i> VSSA, p<0.05, are indicated with *.</p

Strains included in the study.

<p>Legend: V = Vancomycin, T = Teicoplanin, D = Daptomycin, S = Susceptible, R = Resistant.</p

δ-hemolysis, Autolysis Ratio, Percentage of Triton X-100 autolysis <i>vs.</i> VSSA.

<p>Legend:</p><p>*(++) large δ-hemolysis zone; (−/+) low δ-hemolysis zone; (−) no δ-hemolysis zone.</p><p>**measured as the T₀/T₅ O.D. ratio ± SD (Standard Deviation).</p

δ-hemolysis assay of prototype microrganisms.

<p>A) NRS149 (VSSA); B) Mu3 (hVISA); C) Mu50 (VISA).</p

Transcript analysis in presence of vancomycin and daptomycin.

<p>Relative quantitative expression of some autolytic (<i>atl</i>, <i>lyt</i>M), cell-wall turnover (<i>sce</i>D) and cell envelope genes (<i>mpr</i>F, <i>dlt</i>A) of VSSA, hVISA and VISA with VAN (V) and Ca²⁺-DAP (D) <i>vs</i> drug-free conditions (FREE). Statistically significant difference between sample <i>vs.</i> free drug conditions, p<0.05, are indicated with *.</p